WIKI資訊
Protocol for Frozen Sections:Warm 150ml 4% Paraformaldehyde/1x PBS to RT. Fix slides in it, 20 min., RT.1x PBS rinse, 2 times.1x PBS, 30 min., RT. Begin chilling Triton/SSC on ice.0.1% Triton/ 0.1% Sodium Citrate, 2 min., 4°C.All slides: 1x PBS rinse, 2 times (+ 10 min for those non-pos.control slides).(Pos. control slide: in DNase I solution (100μl of 200μg/ml), 10 min., RT. 1x PBS rinse, 2 times in a separate conta......閱讀全文
B) TUNEL in situ procedureB.2.1 Materialsproteinase K (pK) (A2), H2O2 , TdT buffer (A1), TdT enzyme (A2), biotinylated dUTP (A2), TB buffer (A1),
B.3. COMMENTARY B.3.1 Background information The critical steps in the methodology are cell fixation, permeabilization and the concentration
2.3 凋亡細胞的TUNEL和ISNT鑒定的流式細胞儀分析[16]對于培養的細胞，可以將TUNEL或ISNT鑒定同流式細胞儀結合起來分析其發生凋亡的情況。待檢細胞與含有TdT或DNA聚合酶I或Klenow片段及生物素標記的dUTP反應液共孵育一段時間后，加入熒光素（常用FITC）標記的鏈霉抗生物
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox,José C. Rodriguez
In Situ Cell Death (Apoptosis) Detection by TUNEL labelingby Boehringer Mannheim (Catalog No. 1684809), modified by Josiah N. Wilcox andJosé C. Rodrig
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do not go
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do n
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered paraformaldehyde in 100 ml water. Heat and stir (55-60 C – do n
1. INTRODUCTION Apoptosis was observed from invertebrates to lower and higher verterbrates, and intervenes both in physiological and in pat
Materials:Hoechst 33258 (Sigma B-2883).stock: 10 mg/ml in dH20 (40)working dilution: 500μg/ml (50μl stock + 950μl PBS).7-Amino-actinomycin (