WIKI資訊
一、提取抗原蛋白將提取RNA途中留存的樣品，加入150μl 100%酒精充分混勻，靜置5min(RT), 2000×g , 4℃離心5min, 吸取上清至新管中, 加入750μl異丙醇, 混勻, 靜置10min(RT), 12000×g, 4℃離心10min, 棄上清, 加入1ml 0.3mol/L鹽酸胍/95%酒精重懸沉淀, 用加樣器打散沉淀, 混旋20~30秒, 靜置20min(RT) , 7500×g, 4℃離心5min, 棄上清 , 重新加入1ml 0.3mol/L鹽酸胍/95%酒精兩次, 重懸沉淀, 離心后棄上清, 加入100% 無水乙醇 1ml, 混旋1min, 靜置20min(RT), 7500×g, 4℃離心5min,&nbs......閱讀全文
Western免疫印跡（Western Blot）是將蛋白質轉移到膜上，然后利用抗體進行檢測。對已知表達蛋白，可用相應抗體作為一抗進行檢測，對新基因的表達產物，可通過融合部分的抗體檢測。 本文主要通過以下幾個方面來詳細地介紹一下Western Blot技術: 一、原理 二、分類
Western免疫印跡（Western Blot）是將蛋白質轉移到膜上，然后利用抗體進行檢測。對已知表達蛋白，可用相應抗體作為一抗進行檢測，對新基因的表達產物，可通過融合部分的抗體檢測。一、原理與Southern或Northern雜交方法類似，但Western Blot采用的是聚丙烯酰胺凝膠電泳，被
Western 雜交（主要內容如下）Preparing of Protein LysatesWestern BlottingFar Western BlottingSemi Dry BlottingStripping MembranesTrouble Shooting and Others
四、主要步驟 生物秀為您搜集了現階段幾乎網上所有的Western Blot的中英文資料，僅供實驗參考，可以根據自己實驗的實際情況進行調整： Western Blot PROTOCOL： 點擊查看所有相關文章 五、 實驗常見的問題指南 根據問題的類型主要分成以下幾類（以
實驗概要The method provides a blocking with immunizing peptide protocol.實驗原理Non-specific binding of an antibody to proteins other than the antigen can s
實驗概要Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclona
實驗概要Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclona
back to topProtocolStandard vs. Rapid Immunodetection ProceduresThere are two types of protocols for immunodetection: Standard and rapid.Standard vs.
WESTERN BLOT PROTOCOLIn a Western blot, proteins that are separated on polyacrylamide gels on the basis of size are transferred to a membrane for dete
實驗概要Interest in RNA-protein interactions is booming as we begin to appreciate the role of RNA, not just in well-established processes such as tran
實驗室里經常聽到這樣的抱怨：實驗沒做好，實驗沒結果，心情郁悶。但其實靜下心來查找原因，往往是由于操作上面的不認真，或是一些小小的細節沒有注意到，以下是10個實驗室日常小技巧。 主題： 關于質粒提取的小技巧 內容： 現在用質粒提取試劑盒非常方便，而且菌體培
1、質粒DNA提取 提取質粒的時候，最后一步的酒精揮發很關鍵，基本上是其后續的酶切反應的決定性因素。所以這一步盡量揮發長一點時間，最好是空調吹熱風，或是37度溫箱放長一點的時間，我試過室溫過夜，酶切很好。 將amp濃度提高到200ug/ml，下班前接種，次日一早收獲菌體，此時OD雖然不高，質
實驗概要 Western blot detection of histone proteins. 實驗步驟 The following protocol refers to the western blot detection of histone &nbs
PROTOCOLTo 1.5 mL eppendorf tubes add:200 μg of protein extract (see Western blot protocol for protein sample preps)q.s. to 300 μL with RIPA (with pro
實驗概要The method provides a procedure and tips for detecting histone proteins.The protocol refers to the western blot detection of histone p
Yeast DNA PreparationYeast Genomic Preparation (Gottschling Lab)Rapid method for yeast genomic DNA isolation Yeast DNA Preparation (r
最近的預印本雜志bioRxiv發表了德國波恩大學，夏里特大學，德國馬克斯·普朗克精神病研究所等單位聯合研究成果，闡述了SARS-CoV-2病毒可以抑制自噬。亞精胺，MK-2206，氯硝柳胺等作用于自噬的小分子化合物，可以作為抗病毒潛在治療藥物。Analysis of SARS-CoV-2-contr
人胚腎 293 (HEK293) 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體 (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 H
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from con?uent T-?asks or from suspension cultures at h
IntroductionIn our lab we use the term 'cell-free system' when we talk about the examination of apoptotic activity in cytoplasmic extrac
實驗概要The following protocol provides a method of removal of antibodies from western blots.實驗原理Stripping is the term used to describe the removal
This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison
韓春雨團隊和他們的NgAgo-gDNA技術自第一次被報道，就一直倍受關注，近日，方舟子公開發文質疑韓春雨"諾獎級"實驗成果的可重復性問題，(推薦閱讀：方舟子發文質疑韓春雨“諾獎級”實驗成果，你怎么看？）而對于方舟子和部分網友的質疑，韓春雨也于7月2日在百度貼吧上做出了回應。 早前，韓春雨一經報
OUTLINEWestern blot is a wide used technique to identify a target protein/s for the certain antibody.PROTOCOLPrepare platelets.Lyse washed platelets (
· Protein detection (Aberdeen's Lab)The method used to locate the proteins following 2D-
實驗概要Sandwich enzyme-linked immunosorbent assays (ELISAs) involve attachment of a capture antibody to a solid phase support. Samples contai
實驗概要Environmental stresses often trigger rapid generation of reactive oxygen species (ROS) in plants. Excessive amount of ROS can cause damage to
或者第5步和第6步改為丙酮洗:5.加入200ul冰冷的丙酮，用手指輕彈EP管，洗去管底和管壁殘余的TCA。6.15000g,離心10－20分鐘,倒掉上清，將EP管倒扣在吸水紙上輕輕控幾下，除去殘余在管口的液體。樣品是酵母細胞超聲后離心獲得的上清，用Loading buffer溶解蛋白沉淀，始終不
一、熒光素酶報告基因的檢測原理熒光素酶（Luciferase）是生物體內催化熒光素(luciferin)或脂肪醛(firefly aldehyde)氧化發光的一類酶的總稱，來自于自然界能夠發光的生物。自然界存在的熒光素酶來自螢火蟲、發光細菌、發光海星、發光節蟲、發光魚、發光甲蟲等。細菌熒光素酶對熱敏
The siRNA user guide (revised May 6, 2004) Selection of siRNA duplexes from the target mRNA sequenceUsing Drosophila melanogaster lysa