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實驗概要We provide a protocol using an FITC-conjugated primary antibody and a biotinylated primary antibody. Those are in turn recognized by anti-FITC HRP and streptavidin-AP conjugates.主要試劑1. Streptavidin alkaline phosphatase conjugate2. Phosphate buffered saline (10 x concentrate solution). For 1 liter weight : 80 g NaCl; 2 g KH2PO4; 14.4 g Na2HPO42H2O. Add distilled water to 1 liter. Check......閱讀全文
實驗概要Cytokine ELISPOT has become a powerful routine tool for the analysis of disease- as well as vaccine-induced T-cell responses. The meth
實驗概要During more than 25 years of application in immunological sciences, ELISPOT has been established as a routine, robust, versatile, and
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T CellsINTRODUCTIONThe enzyme-linked immunospot (ELISPOT) assay
21. Add 100 μL of developing buffer to all wells using a multichannel pipette and incubate at room temperature for at least 2
主要試劑1. Blocking reagent after coating: 10% fetal calf serum (FCS) in phosphate-buffered saline (PBS), pH 7.4.2. Cells suspended in complete culture me