細胞周期試劑盒CellcyclestainingKit中文操作步驟
試劑盒特點：1、獨創的活細胞一步法檢測，收集——洗滌——染色，簡單快速 2、兼容固定細胞檢測，改進的固定方法，更少細胞粘連，更小CV值 3、兼容流式和熒光顯微鏡分析，提供詳細protocol固定細胞處理：1、收集細胞懸液，細胞數為2 × 10^5 ~ 1 × 10^6。離心沉淀細胞，去上清，輕敲試管，用剩余液體重懸沉淀；加入室溫的PBS1ml； 2、將所有的細胞緩慢滴入3ml的冰乙醇（-20℃）中，邊滴邊高速渦旋振蕩。該細胞放在-20℃可保存幾個月。3、離心沉淀細胞，棄去乙醇，輕敲試管使沉淀松散，加入室溫PBS 2~5ml，靜置15min使細胞再水合。離心棄上清。 4、加入1 mL的試劑A，渦旋混勻5~10s。室溫孵育30min。活細胞處理：1、收集細胞懸液，細胞數為2 × 10^5 ~ 1 × 10^6。離心沉淀細胞，去上清。用PBS洗一次，棄上清。 2、加入1 mL的試劑A和10μL 的試劑B，渦......閱讀全文
細胞周期試劑盒Cell－cycle－staining－Kit中文操作步驟
試劑盒特點：1、獨創的活細胞一步法檢測，收集——洗滌——染色，簡單快速 ? 2、兼容固定細胞檢測，改進的固定方法，更少細胞粘連，更小CV值 ? 3、兼容流式和熒光顯微鏡分析，提供詳細protocol固定細胞處理：1、收集細胞懸液，細胞數為2 × 10^5 ~ 1 × 10^6。離心沉淀細胞，去上清，
FIXATION－and－DNA－Staining－for－Cell－Cycle－Analysis
Background This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to e
Cell－Cycle－Staining－ProtocolDAPI
1. Harvest cells- wash 2X in PBS to get rid of serum proteins. 1200rpm, 5 min2. Resuspend pellet (up to 3x106 cells) in 1.2 ml PBS (Ca and Mg free).3.
細胞計數試劑盒(Cell－Counting－Kit)操作方法
一、制作標準曲線（測定細胞具體數量時）1、先用細胞計數板計數所制備的細胞懸液中的細胞數量，然后接種細胞。2、按比例（例如：1/2比例）依次用培養基等比稀釋成一個細胞濃度梯度，一般要做3-5個細胞濃度梯度，每組3-6個復孔。3、接種后培養2-4小時使細胞貼壁，然后加CCK試劑培養一定時間后測定OD值，
DNA－Cell－Cycle
Solutions70% ethanolribonuclease (100 μg/ml DNase free, Sigma)propidium iodide ( 50 μg/ml in PBS)ProcedureHarvest cells. Spin at 1200 rpm for 5 minute
CELL－CYCLE－ANALYSIS
PROPIDIUM IODIDE: The most commonly used dye for DNA content/cell cycle analysis is PROPIDIUM IODIDE (PI). It can be used to stain whole cells or isol
Staining－Methods－for－cell
death Z. Xia 10/2/95 The simplest way: trypan blue.? Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells;?
Cyclins－and－Cell－Cycle－Regulation
The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins whi
Staining－Methods－for－cell－death
The simplest way: trypan blue.? Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells;? P.I. (propidium iodide)-r
Flow－Cytometric－Analysis－of－Cell－Cycle
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells
Yeast－Cell－Cycle－by－Flow－Cytometry
ReagentsCold absolute ethanol.0.5 M Na citrate stock (filtered), 50mM diluted stock.10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C).4
Cell－Surface－Immunofluorescence－Staining－Protocol
實驗概要A method of identifying ?and enumerating specific cell types in a heterogeneous population of ?cells by enhancing the specific staining of desired
Vybrant?－DyeCycle?－Ruby－stain
實驗概要Live cell studies ?of cellular DNA content and cell cycle distribution are useful to detect ?variations of growth patterns due to a variety of phy
PTEN－dependent－cell－cycle－arrest－and－apoptosis
PTEN is a tumor suppressor gene. Recombinant PTEN is capable of dephosphorylating phosphatidylinositol 3,4,5-triphosphate[PI(3,4,5)P3], the product of
Cell－cycle－analysis－of－Escherichia－coli－cells
Cell cycle analysis of?Escherichia coli?cellsC period = the time for a round of chromosome replicationD period = the time between the end of a round o
BTG－family－proteins－and－cell－cycle－regulation
BTG2 is found to be one of the immediate early genes up-regulated by neural growth factor (NGF) and epidermal growth factor (EGF). Its transcriptional
Vybrant?－DyeCycle?－Violet－Stain
實驗概要Live cell studies ?of cellular DNA content and cell cycle distribution are useful to detect ?variations of growth patterns due to a variety of phy
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis 1.1 Introduction 2 1.1.1 Terminology of cell death 2 1.1.2 Differences between necros
Regulation－of－cell－cycle－progression－by－Plk3
The focus of this pathway is to illustrate the role of Polo-like Kinase 3 (Plk3 also known as Prk and Fnk) as part of the regulatory cascade leading t
Cell－Cycle:－G2/M－Checkpoint
The G2/M DNA damage checkpoint prevents the cell from entering mitosis (M phase) if the genome is damaged. The Cdc2-cyclin B kinase is pivotal in regu
CellTrace?－CFSE－Cell－Proliferation－Kit
實驗概要The CellTrace? ?CFSE Cell Proliferation Kit provides a versatile and well-retained ?cell-tracing reagent in a convenient and easy-to-use form. The
Cell－Cycle：細胞永生化研究進展
每一天，你身體內的一些細胞會停止分裂，這是一件好事。而有些細胞會無限增殖，這是大多數惡性腫瘤發展的一個重要早期步驟。 盡管細胞無限增殖在癌癥中有著非常的重要性，但研究人員一直對于細胞永生化背后的分子機制知之甚少。這是因為科學家缺乏好方法研究永生化人類細胞。在發表在Cell Cycle 雜志上的
Cell－Cycle:－G1/S－Check－Point
The G1/S cell cycle checkpoint controls the passage of eukaryotic cells from the first 'gap' phase (G1) into the DNA synthesis phase (S). Two
Cell－Cycle－－J－Neurosci：脊髓損傷引發大腦損害
很多脊髓損傷的研究都集中于揭示脊髓損傷所引發的效應，然而往往在研究中科學家們很容易忽視脊髓損傷對大腦所引發的效應。近日，來自馬里蘭大學的研究人員通過研究首次揭示，脊髓損傷（SCI）可以促進廣泛和持續的大腦炎癥，進而引發神經細胞的逐漸缺失以及認知問題和抑郁癥等。 相關研究發表于國際雜志Journ
Regulation－of－p27－Phosphorylation－during－Cell－Cycle－Progression
p27/Kip1 regulates the cell cycle by inhibiting the checkpoint kinase cdk2/cyclin E and blocking cell cycle progression through the G1-S transition. C
Vybrant?－DyeCycle?－Green－and－Orange－Stains
實驗概要Live ?cell studies of cellular DNA content and cell cycle distribution are ?useful to detect variations of growth patterns due to a variety of ?ph
Cell－Staining－for－SenescenceAssociated－betaGalactosidase－－Activity
Description Cell Staining for Senescence-Associated a-Galactosidase (SA-a-Gal) Activity? Procedure 1. Carefully remove the growth media from the cel
Detection－of－Intracellular－Antigens－by－Flow－Cytometry
實驗概要Fix and Perm ?reagents are designed for use with all commercially available flow ?cytometers. Alignment and compensation should be performed accor
LIVE/DEAD?－Violet－Viability/Vitality－Kit
實驗概要The LIVE/DEAD? ?Violet Viability/Vitality Kit provides a two-color fluorescence cell ?viability and vitality assay that is based on the simultaneo
使用CCCadvanced?FN1無異源耗材培養人多能干細胞（二）
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI?, USA) were initially thawed and pre-cultivat